二甲双胍治疗可能提高女性多囊卵巢综合征网膜素-1水平
2010-12-02 00:00:00来源：医脉通阅读：116次
文献标题：Metformin treatment may increase omentin-1 levels in women with polycystic ovary syndrome.
文献出处：Diabetes. 2010 ;59(12):3023-31.
期刊影响因子：8.505
多囊卵巢综合征(PCOS)与代谢综合征相关。网膜素-1（omentin-1）水平降低与肥胖和糖尿病有关。本文研究二甲双胍治疗对多囊卵巢综合征患者omentin-1水平的影响及omentin-1对体外迁移与血管生成的效果。
采用酶联免疫吸附法(ELISA)测定血清omentin-1水平。在通过研究生长因子减少的基底膜上的人微血管内皮细胞(HMEC-1)毛细血管形成评估血管生成。在改良的博伊登室(进行趋化性实验的装置)中测定内皮细胞迁移。利用稳定转染的HMEC-1细胞（含有5个NF-кB重复结合位点连锁荧光素酶报告基因的acis-报告质粒）来研究核因子κB (NF-κB)。。使用蛋白印迹法评估AKT磷酸化。
结果发现，多囊卵巢综合征患者血清omentin-1明显降低(P < 0.05)。二甲双胍治疗6个月后，血清omentin-1明显升高(P < 0.01)。重要的是，hs-CRP变化与血清omentin-1的变化呈明显负相关(P = 0.036). 与对照组相比，PCOS女性血清中体外迁移和血管生成显著增加(P < 0.01);二甲双胍治疗似乎是通过NF-κB 和 Akt信号通道调节omentin-1水平从而明显减弱这一效应(P < 0.01) 。omentin-1可以明显减弱C反应蛋白和血管内皮生长因子诱导的体外迁移和血管生成。
研究者认为，提高omentin-1水平可能会起作用但不足以解释二甲双胍治疗的多囊卵巢综合征患者血清的炎症反应及血管生成减少。
医脉通推荐英文摘要
Diabetes. 2010 Dec;59(12):3023-31.
Metformin treatment may increase omentin-1 levels in women with polycystic ovary syndrome.
Tan BK, Adya R, Farhatullah S, Chen J, Lehnert H, Randeva HS.
OBJECTIVE Polycystic ovary syndrome (PCOS) is associated with the metabolic syndrome. Decreased omentin-1 levels are associated with obesity and diabetes. To study the effects of metformin treatment on omentin-1 levels in PCOS subjects and effects of omentin-1 on in vitro migration and angiogenesis.
RESEARCH DESIGN AND METHODS Serum omentin-1 was measured by ELISA. Angiogenesis was assessed by studying capillary tube formation in human microvascular endothelial cells (HMEC-1) on growth factor reduced Matrigel. Endothelial cell migration assay was performed in a modified Boyden chamber. Nuclear factor-κB (NF-κB) was studied by stably transfecting HMEC-1 cells with a cis-reporter plasmid containing luciferase reporter gene linked to five repeats of NF-κB binding sites. Akt phosphorylation was assessed by Western blotting.
RESULTS Serum omentin-1 was significantly lower in PCOS women (P < 0.05). After 6 months of metformin treatment, there was a significant increase in serum omentin-1 (P < 0.01). Importantly, changes in hs-CRP were significantly negatively correlated with changes in serum omentin-1 (P = 0.036). In vitro migration and angiogenesis were significantly increased in serum from PCOS women (P < 0.01) compared with matched control subjects; these effects were significantly attenuated by metformin treatment (P < 0.01) plausibly through the regulation of omentin-1 levels via NF-κB and Akt pathways. CRP and VEGF induced in vitro migration, and angiogenesis was significantly decreased by omentin-1.
CONCLUSIONS Increases in omentin-1 levels may play a role but are not sufficient to explain the decreased inflammatory and angiogenic effects of sera from metformin-treated PCOS women.
文献来源
Tan BK, et al. Metformin treatment may increase omentin-1 levels in women with polycystic ovary syndrome. Diabetes. 2010 Dec;59(12):3023-31. [期刊链接|PubMed链接]